It provides a longer range attack, and two mutation points when mutating a soldier which allows for faster mutation especially in Hero Mode.
In Hero Mode, mutants still needs two hits to evolve into Berserk level because the Nano Hook only provide them four "small" mutation points - however, one can resort to infecting one soldier then take damage to evolve. In Hero Mode X, if the players become the Xeno / Rook / Armored Mutant / Devil Terminator, the Nano Hook is not available to use, but he can still earn 2 mutation points for each soldier infected.
- If the item is equipped by Dread, the HUD will show GR SWAT contamination instead of the Nano Knife. This is not applied to Smoke Nano, as his left hand is still shown with a drill. This glitch has been fixed after Dread is upgraded to 2.0 version.
- As a knife, Nano Hook deals 100 HP damage via LMB and 200 HP via RBM.
- In CF Russia, if a Smoke Nano equips the Nano Hook, it will display as KMB-317 Hook, instead of Hook.